The Effect Of Ph On Enzymatic Reaction

Title : Factor Affecting Enzyme Activity (pH) Objectives 1. To read the following(a) book of instructions carefully. 2. To determine the significant effects of different pH levels on enzyme activities. 3. Find out the optimal pH execute for the reactions of the presumption enzyme through a plot graph. Variables Independent variables : pH of re resultant role in streak tube Dependent variables : Time for fade of colour of the solution Constant variables : Total volume of solution in distributively test tube, volume of stiffen in apiece test tube, temperature of the solution. Materials Beakers, test tubes in a rack, dropper, grading pen, pipettes, sucker, global indicator paper, stop watch, distilled water, fresh starch solution, single solution, amylase, 0,1mol/L hydrochloric astringent (pH=1), 0.1mol/L acetic venereal infection (pH=3), 0.001mol/L sodium hydroxide (pH=11), 0.1 mol/L sodium hydroxide (pH=13), trumpery rod, grooved white t ile. Procedures 1. The pH of both tart and two basic was recorded in the table below. 2. 5 test tubes with the solution were set up as shown as in the table below. 3. The contents of each test tubes were entangled and the pH was checked. It was written down to the right of the first in the pH column if the pH is different from the pH recorded. 4. every the tubes were find ad the time when the blue colour disappeared in each tube was noted. 5. The reciprocal of each time was metric and the harbor was inserted in the 1/time column. This was used as the exercise of the enzyme. 6. A graph of activity (1/time) against pH was plotted on a graph paper. Results Test tubes |mL starch |mL iodine |mL amylase |mL distilled water |mL of acid or basic |pH |Time for slicing of colours, t (s) |1/time (s-1) | | | | | | | |Before |After | | | |1 |3 |3 |3 |1 |0 |7 |6 |cl |0.0067 | |2 |3 |3 |3 |0 |1 mL of 0.1 mol/L HCL |1 |3 |240 |0.0042 | |3 |3 |3 |3 |0 |1 mL of 0.1 mol/L acetic acid |3 |5 |195 |! 0.0051 | |4 |3 |3 |3 |0 |1 mL of 0.001...If you want to get a full essay, put up it on our website: OrderCustomPaper.com

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